Involverte: Norges Laboratorium for Dopinganalyse, Norges idrettshøgskole
Beskrivelse: Human Growth Hormone (hGH) acts an anabolic effect on skeletal muscle tissue by increasing the influx of amino acids into the muscle cells, and increasing the speed of the protein synthesis. It is also essential for normal growth and development of different organs and tissues. These properties have made rhGH a common doping agent, even though the ergogenic potential is a matter of discussion.
The current direct method for detecting rhGH has a window of detection of only 24 hours, which consequently limits its effectiveness. A method for rhGH detection using GH-dependent markers such as Insulin-like Growth Factor 1 (IGF-1) and Pro-Collagen type 3 Peptide (P-III-P) has been suggested (Wallace et al., 1999). These growth factors have been shown to have a dose dependent response to the administration of rhGH, and to be resistive to the acute effect of physical activity. The problem with this method is however the great inter-individual variance, making it difficult to discriminate between natural variance and doping (Gibney J et al., 2007).
The problem of large inter-individual variance can potentially be circumvented by using individual values as references, applying the biological profiling idea – similar to blood and testosterone profiles - in the detection of abuse of rhGH. This project aims to investigate the intra-individual variance and robustness of IGF-1 and P-III-P, exploring the possibilities of using individual profiles as a method for detecting the use of rhGH in Sports. To test whether IGF-1 and P-III-P levels are affected by physical activity in individual profiles, we will study the profiles of markers in top level athletes in periods of rest and after strenuous exercise.
For this purpose, the IGF-1 and P-III-P profiles of four different groups will be collected during 1, 5 years. The groups will consist of 20 power sports athletes, 20 endurance athletes, 10 body builders and a control group of 20 sedentary persons.
Status: Fullført (intern rapport)